Methyl jasmonate reduces the inflammation and apoptosis of HK-2 cells induced by LPS by regulating the NF-κB pathway

Background: Methyl jasmonate is a bioactive oxylipid that participates in the defense-related mechanisms of plants. The anti-inflammatory and anti-oxidative capacities methyl against lipopolysaccharide (LPS) induced arthritis have been widely investigated. However, role LPS-induced cell model tubular-interstitial nephritis (TIN) has not reported. Methods: LPS (5 µg/mL) was applied to treat human renal tubular epithelial line (HK-2) for establishment TIN model. HK-2 incubated with 10 or 20 µM jasmonate, viability apoptosis were assessed by MTT flow cytometry. ELISA qRT-PCR performed determine levels interleukin (IL)-1 beta (IL-1β), IL-6, IL-8 tumor necrosis factor-α (TNF-α). downstream pathway investigated western blot. Results: cytotoxicity accompanied decrease increase apoptosis. dosage dependently enhanced reduced ameliorate cytotoxicity. also inflammatory response increased IL-1β, TNF-α. attenuated inflammation cell. Protein expression IκBα down-regulated, p65 phosphorylation up-regulated HK-2. Conclusion: demonstrated anti-apoptotic effects on through suppression NF-κB activation.